How the tissue samples were obtained in the field.


    Only a small bit of meristematic tissue is required to extract the DNA.



    After measuring the size of the crown a pocket knife was used to remove a small sample of tubercle.




    The blade was wiped clean with a paper towel and then with an alcohol wipe in between each sample.



    Each tissue sample then went into a snack-sized ziplock bag with the location and specimen number recorded on the bag with a permanent marker.
        After being collated by specimen number they were placed in a larger plastic ziplock bag and stored on ice in a cooler until we reached the lab at UNAM.



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